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The fluorescence sensor for saccharide based on internal conversion.

Xiang-Ying Sun·Bin Liu
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Abstract

In this paper, an internal conversion (IC) fluorescence probe N-(o-boronic acid)benzyl-1-naphthylamine (BBNA) was prepared from 1-naphthylamine and 2-formylbenzeneboronic acid. The fluorescence parameters of BBNA were investigated in a variety of solvents. When BBNA interacted with D-fructose in phosphate buffer solution of 30% MeOH, pH 8.21 (v/v), the fluorescence intensity increased and emission maximum red-shifted slightly with increasing D-fructose concentration. In the presence of D-fructose, the fluorescence quantum yield of BBNA increased with increasing solvent polarity, suggesting that internal conversion (IC) occurred with BBNA. The binding force of BBNA with d-fructose was the strongest, and the stability constant (K) of D-fructose was 99.9 mol/L. Therefore, a selective recognition system based on IC was constructed for D-fructose.

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1-NaphthylamineBoronic AcidsFluorescent DyesFructoseGlucansHydrogen-Ion ConcentrationMolecular StructurePhotochemistrySpectrometry, FluorescenceJournal ArticleResearch Support, Non-U.S. Gov't

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Xiang-Ying Sun, Bin Liu. The fluorescence sensor for saccharide based on internal conversion.. https://doi.org/10.1002/bio.841

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