PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 16135448

[Experimental techniques for ancient DNA research].

Shu-juan Yang·Xu-long Lai·Xian-hua Tang·Gui-lian Sheng
View original source ↗Export metadata ↓

Abstract

The development of modern molecular biological techniques makes it possible to study minimum DNA from ancient materials. During past decade,a lot of significant achievements on ancient DNA research have been made in many fields especially in molecular evolutionary biology. The nature of degradation and contamination of ancient DNA from ancient biological materials pose a dominating problem in ancient DNA research. Therefore,the experiments should be modified based on the modern molecular techniques and more factors should be considered when the results are analyzed. In this paper,authors review the general experimental protocols on sampling,extraction and amplification as well as authenticity of ancient DNA.

Explore related subjects

English AbstractJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Shu-juan Yang, Xu-long Lai, Xian-hua Tang, Gui-lian Sheng. 2002. [Experimental techniques for ancient DNA research].. https://pubmed.ncbi.nlm.nih.gov/16135448/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

[Why Danes are smug--a comparative study of life satisfaction in the EU].

Danes are by far the most content citizens in the EU. We test a number of (im)plausible hypotheses and find that the solid depth of Danish well-being is multifactorial, but with two decisive factors: winning the 1992 European Championship in football and having constantly low (and undoubtedly realistic) expectations for the coming year. With an undoubtedly disappointing 2007 in store, we do not wish to foster false hopes. However, some tepid comfort might perhaps be taken from the fact that if you lower your expectations enough, you might feel a bit better next New Year.

English Abstract↗
PubMed2006

[The anatomy lesson of Dr Nicolaes Tulp by Rembrandt (1632) and the findings during dissection of the forearm of a cadaver: anatomical discrepancies].

OBJECTIVE: To compare The anatomy lesson of Dr Nicolaes Tulp by Rembrandt (1632) with the findings during dissection of the forearm of a cadaver. DESIGN: Descriptive. METHOD: The left forearm of a 41-year-old white male cadaver was dissected, photographs being taken at every stage of dissection. The anatomical structures in the original painting and during dissection were described and compared. At dissection, several structures were displaced in an attempt to reproduce the anatomical structures as shown in the painting. RESULTS: Dissection revealed four anatomical differences in comparison with Rembrandt's painting: (a) the muscle belly seen at the proximal ulnar side of the forearm in the painting was not seen on dissection; section of the insertion of the M. flexor carpi radialis and transposition of this muscle to the location of the muscle belly created the possibility of lifting up the M. flexor digitorum superficialis and reproducing the muscle belly; (b) in the painting, Tulp lifted only the M. flexor digitorum superficialis, but the amount of muscle tissue found in the forceps was increased in the painting compared with the dissection; (c) the positions of the bellies of the M. flexor digitorum superficialis to the index finger and 5th digit and to the 3rd and 4th digits were found to be reversed; (d) the longitudinal white structure situated in the ulnar part of the 5th digit in the painting was not found on dissection. This may have been an anatomical variant of the ulnar nerve. CONCLUSION: The anatomical characteristics of the painting could not be reproduced by dissection of the forearm of a cadaver.

English Abstract↗
PubMed2006

[Developing COPD--25 years follow-up study of the general population].

Smokers are more prone to developing chronic obstructive pulmonary disease (COPD) than non-smokers. This knowledge comes from studies spanning 10 years or less. We used data from The Copenhagen City Heart Study to determine the 25-year absolute risk of developing COPD in healthy individuals. The 25-year incidence of moderate and severe COPD was 20.7% and 3.6%, respectively. Smoking cessation, especially early in the course of the follow-up, decreased the risk substantially. The absolute risk of developing COPD among smokers is at least 25%, which is higher than previously anticipated.

English Abstract↗