The minimal functional sequence of protamine.

Jun Feng Liang; Victor C Yang; Yekaterina Vaynshteyn

doi:10.1016/j.bbrc.2005.08.151

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The minimal functional sequence of protamine.

Jun Feng Liang·Victor C Yang·Yekaterina Vaynshteyn

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Abstract

Despite its nearly universal applications, protamine, a mixture of four major peptides with different sequences, is associated with clinically significant side effects. Through a well-designed enzyme digestion method, various low molecular weight protamine peptides were obtained. Among them, two low molecular weight protamine peptides with the same or even more potent heparin neutralization abilities as native protamine were identified through both in vitro and in vivo tests. In addition, in vivo experiments showed that compared to native protamine, these two low molecular weight protamine peptides were less toxic and would be safer for clinical use.

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Amino Acid Sequence Animals Blood Coagulation Blood Platelets Dose-Response Relationship, Drug Female Heparin Humans Molecular Sequence Data Protamines Protein Conformation Rats Rats, Sprague-Dawley Structure-Activity Relationship Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

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Jun Feng Liang, Victor C Yang, Yekaterina Vaynshteyn. 2005-10-21. The minimal functional sequence of protamine.. https://doi.org/10.1016/j.bbrc.2005.08.151

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