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Ionic liquid-based aqueous two-phase system, a sample pretreatment procedure prior to high-performance liquid chromatography of opium alkaloids.

Shehong Li·Chiyang He·Huwei Liu·Kean Li·Feng Liu
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Abstract

An ionic liquid, 1-butyl-3-methylimidazolium chloride ([C4 mim]Cl)/salt aqueous two-phase systems (ATPS) was presented as a simple, rapid and effective sample pretreatment technique coupled with high-performance liquid chromatography (HPLC) for analysis of the major opium alkaloids in Pericarpium papaveris. To find optimal conditions, the partition behaviors of codeine and papaverine in ionic liquid/salt aqueous two-phase systems were investigated. Various factors were considered systematically, and the results indicated that both the pH value and the salting-out ability of salt had great influence on phase separation. The recoveries of codeine and papaverine were 90.0-100.2% and 99.3-102.0%, respectively, from aqueous samples of P. papaveris by the proposed method.

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Chemical FractionationChromatography, High Pressure LiquidCodeineImidazolesPapaverPapaverinePhosphatesPotassium CompoundsTemperatureJournal ArticleResearch Support, Non-U.S. Gov'tValidation Study

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Shehong Li, Chiyang He, Huwei Liu, Kean Li, Feng Liu. 2005-09-06. Ionic liquid-based aqueous two-phase system, a sample pretreatment procedure prior to high-performance liquid chromatography of opium alkaloids.. https://doi.org/10.1016/j.jchromb.2005.08.005

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