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Construction and direct immunization of eukaryotic expression plasmid encoding human soluble B lymphocyte stimulator.

Guangyu Chen·Hongwu Du·Shanyun Peng·Donggang Xu·Jiaxi Wang
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Abstract

B lymphocyte stimulator of the tumor-necrosis-factor family (BLyS) enhances B cell survival, a function indispensable to B cell maturation. This factor plays a crucial role in enhancing immune responses. Here, to study the primary immune effect of BLyS gene in animals, we constructed recombinant eukaryotic expression plasmid of the human soluble BLyS by using expression vector pcDNA3.1 (-). The recombinant plasmid pcDNA3.1BLyS was injected subcutaneously into BALb/C mice and this administration induced the sustained expression of human BLyS and specific IgG against the recombinant BLyS protein. This study shows that recombinant eukaryotic expression plasmid, pcDNA3.1BLyS, might be used in human gene therapy.

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AnimalsAntibodiesB-Cell Activating FactorB-LymphocytesCytokinesEnzyme-Linked Immunosorbent AssayHumansImmune SystemImmunoglobulinsMembrane ProteinsMiceMice, Inbred BALB CModels, StatisticalPlasmidsReceptors, Tumor Necrosis FactorRecombinant ProteinsTime FactorsTumor Necrosis Factor-alphaJournal ArticleResearch Support, Non-U.S. Gov't

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Guangyu Chen, Hongwu Du, Shanyun Peng, Donggang Xu, Jiaxi Wang. 2006-01-01. Construction and direct immunization of eukaryotic expression plasmid encoding human soluble B lymphocyte stimulator.. https://doi.org/10.2741/1784

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