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PubMed · 16149042

Probing lectin and sperm with carbohydrate-modified quantum dots.

Anandakathir Robinson·Jim-Min Fang·Pi-Tai Chou·Kuang-Wen Liao·Rea-Min Chu·Shyh-Jye Lee
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Abstract

We report the encapsulation of quantum dots with biologically important beta-N-acetylglucosamine (GlcNAc) in different ratios, together with studies of their specific/sensitive multivalent interactions with lectins and sperm by fluorimetry, transmission electron microscopy, dynamic light scattering microscopy, confocal imaging techniques, and flow cytometry. These GlcNAc-encapsulated quantum dots (QDGLNs) specifically bind to wheat germ agglutinin, and cause fluorescence quenching and aggregation. Further studies of QDGLNs and the mannose-encapsulated QDs (QDMANs) with sperm revealed site-specific interactions, in which QDGLNs bind to the head of the sperm, while QDMANs spread over the whole sperm body.

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AcetylglucosamineAnimalsFlow CytometryFluorometryLightMaleMicroscopy, ConfocalMicroscopy, Electron, TransmissionQuantum DotsScattering, RadiationSperm HeadSpermatozoaSwineWheat Germ AgglutininsJournal ArticleResearch Support, Non-U.S. Gov't

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Anandakathir Robinson, Jim-Min Fang, Pi-Tai Chou, Kuang-Wen Liao, Rea-Min Chu, Shyh-Jye Lee. 2005. Probing lectin and sperm with carbohydrate-modified quantum dots.. https://doi.org/10.1002/cbic.200500112

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