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PubMed · 16156901

Rapid single nucleotide polymorphism mapping in C. elegans.

M Wayne Davis·Marc Hammarlund·Tracey Harrach·Patrick Hullett·Shawn Olsen·Erik M Jorgensen
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Abstract

BACKGROUND: In C. elegans, single nucleotide polymorphisms (SNPs) can function as silent genetic markers, with applications ranging from classical two- and three-factor mapping to measuring recombination across whole chromosomes. RESULTS: Here, we describe a set of 48 primer pairs that flank SNPs evenly spaced across the C. elegans genome and that work under identical PCR conditions. Each SNP in this set alters a DraI site, enabling rapid and parallel scoring. We describe a procedure using these reagents to quickly and reliably map mutations. We show that these techniques correctly map a known gene, dpy-5. We then use these techniques to map mutations in an uncharacterized strain, and show that its behavioral phenotype can be simultaneously mapped to three loci. CONCLUSION: Together, the reagents and methods described represent a significant advance in the accurate, rapid and inexpensive mapping of genes in C. elegans.

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AnimalsCaenorhabditis elegansChromosome MappingDNA PrimersFemaleGenetic LinkageGenetic MarkersGenomeMaleModels, GeneticMutationNucleotide MappingPhenotypePhysical Chromosome MappingPolymerase Chain ReactionPolymorphism, Single NucleotideRecombination, GeneticJournal Article

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M Wayne Davis, Marc Hammarlund, Tracey Harrach, Patrick Hullett, Shawn Olsen, Erik M Jorgensen. 2005-09-12. Rapid single nucleotide polymorphism mapping in C. elegans.. https://doi.org/10.1186/1471-2164-6-118

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