Rapid affinity purification processes for cyclodextrin glycosyltransferase from Bacillus circulans.

A Rosso; S Ferrarotti; M V Miranda; N Krymkiewicz; B C Nudel; O Cascone

doi:10.1007/s10529-005-8654-6

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Rapid affinity purification processes for cyclodextrin glycosyltransferase from Bacillus circulans.

A Rosso·S Ferrarotti·M V Miranda·N Krymkiewicz·B C Nudel·O Cascone

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Abstract

Two rapid and easy-to-scale-up methods for the purification of cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans were developed: affinity precipitation with starch and aqueous two-phase partition. The first method, optimised by a factorial design, gave an 80% CGTase adsorption at 11% starch and 1.6% ammonium sulphate, and a 65% recovery after elution with 10 mM alpha-cyclodextrin. The purification factor was 17. Aqueous two-phase partition yielded a 72% CGTase recovery in a two-step procedure; CGTase was obtained in the bottom phase with a purification factor of 37.

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Bacillus Chromatography, Affinity Glucosyltransferases Species Specificity Starch Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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A Rosso, S Ferrarotti, M V Miranda, N Krymkiewicz, B C Nudel, O Cascone. 2005. Rapid affinity purification processes for cyclodextrin glycosyltransferase from Bacillus circulans.. https://doi.org/10.1007/s10529-005-8654-6

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