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PubMed · 1616703

A rapid method for cosmid cloning.

M G Loftus·L M Foster·I K Ross
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Abstract

We present a method for genomic library construction using cosmid vectors. With a combination of backfilling with Klenow enzyme and a cosmid vector with two cos sites, a DNA bank in excess of 500,000 clones can be made from 10 micrograms of genomic DNA. The method is more rapid than conventional protocols because size fractionation of target DNA is not necessary. A further advantage is that libraries can be made from relatively small amounts of genomic DNA.

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Cloning, MolecularCosmidsDNADNA Polymerase IDNA, FungalGenomic LibraryJournal ArticleResearch Support, Non-U.S. Gov't

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M G Loftus, L M Foster, I K Ross. 1992. A rapid method for cosmid cloning.. https://pubmed.ncbi.nlm.nih.gov/1616703/

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