Immunization with a gene encoding granulocyte-macrophage colony-stimulating factor inserted with a single helper T-cell epitope of an intracellular bacterium induces a specific T-cell subset and protective immunity.

Toshi Nagata; Masato Uchijima; Hiroshi Uchiyama; Takashi Yamada; Taiki Aoshi; Yukio Koide

doi:10.1016/j.vaccine.2005.08.072

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Immunization with a gene encoding granulocyte-macrophage colony-stimulating factor inserted with a single helper T-cell epitope of an intracellular bacterium induces a specific T-cell subset and protective immunity.

Toshi Nagata·Masato Uchijima·Hiroshi Uchiyama·Takashi Yamada·Taiki Aoshi·Yukio Koide

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Abstract

We evaluated here the effect of immunization with a gene encoding granulocyte-macrophage colony-stimulating factor (GM-CSF) inserted with a helper T cell (Th) epitope, listeriolysin O (LLO) 215-226 derived from Listeria monocytogenes on induction of a specific Th by gene gun bombardment. Immunization of C3H/He mice with pGM215m plasmid encoding murine GM-CSF inserted with LLO 215-226 Th epitope gave the epitope-specific proliferative responses of CD4(+) T lymphocytes. In addition, specific interferon-gamma production from the splenocytes was observed. Concomitantly, pGM215m-immunized mice showed significant protective immunity against lethal listerial challenge. These results suggest that immunization of a gene for GM-CSF inserted with a Th epitope is useful for eliciting a specific Th subset in vivo.

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Animals Base Sequence CD4-Positive T-Lymphocytes DNA Primers Enzyme-Linked Immunosorbent Assay Epitopes Granulocyte-Macrophage Colony-Stimulating Factor Interferon-gamma Listeria monocytogenes Mice Mice, Inbred C3H Plasmids T-Lymphocyte Subsets T-Lymphocytes, Helper-Inducer Journal Article Research Support, Non-U.S. Gov't

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Toshi Nagata, Masato Uchijima, Hiroshi Uchiyama, Takashi Yamada, Taiki Aoshi, Yukio Koide. 2005-09-06. Immunization with a gene encoding granulocyte-macrophage colony-stimulating factor inserted with a single helper T-cell epitope of an intracellular bacterium induces a specific T-cell subset and protective immunity.. https://doi.org/10.1016/j.vaccine.2005.08.072

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