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Nucleic acid amplification-based techniques for pathogen detection and identification.

Paul T Monis·Steven Giglio
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Abstract

Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed.

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DNA, BacterialDNA, ViralMolecular BiologyMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesNucleic Acid ConformationNucleic Acid HybridizationVirulence FactorsJournal ArticleReview

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Paul T Monis, Steven Giglio. 2005-09-19. Nucleic acid amplification-based techniques for pathogen detection and identification.. https://doi.org/10.1016/j.meegid.2005.08.004

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