New capabilities in determining the binding parameters for ligand-receptor interaction.

Sergey Bobrovnik

doi:10.1016/j.jbbm.2005.08.003

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New capabilities in determining the binding parameters for ligand-receptor interaction.

Sergey Bobrovnik

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Abstract

A new method for determining the binding parameters of ligand-receptor interaction is suggested. The method is based on the application of the so-called coordinate of dilution, suggested by us earlier. We demonstrated that it is possible to determine the binding characteristics of ligand-receptor interaction using either the measurement of the concentration of the ligand-receptor complex at a state of equilibrium or the concentration of free receptors at different dilutions of the studying ligand-receptor mixture. The method also allows the determination of the concentration of the ligand in a pre-existing ligand-receptor mixture without preliminary separation of the interacting counterparts. For this reason the suggested method could be especially useful when the studying very labile receptors for which purification from the corresponding ligand is very difficult or impossible.

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Algorithms Antigen-Antibody Complex Enzyme-Linked Immunosorbent Assay Kinetics Ligands Models, Chemical Protein Binding Protein Interaction Mapping Receptors, Cell Surface Journal Article

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Sergey Bobrovnik. 2005-09-13. New capabilities in determining the binding parameters for ligand-receptor interaction.. https://doi.org/10.1016/j.jbbm.2005.08.003

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