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PubMed · 16190439

Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform.

Markus Burkhardt·Katrin G Heinze·Petra Schwille
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Abstract

We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allows for accommodation of up to 15 detection channels covering the entire visible spectral range. As a proof of principle, we successfully demonstrate simultaneous FCS of four distinct fluorescent quantum dot species being mixed in aqueous solution. Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complexbiomolecular dynamics on a single molecule level.

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Equipment DesignEquipment Failure AnalysisFeasibility StudiesImage EnhancementImage Interpretation, Computer-AssistedMicroscopy, ConfocalMicroscopy, Fluorescence, MultiphotonRefractometrySpectrometry, FluorescenceEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Markus Burkhardt, Katrin G Heinze, Petra Schwille. 2005-09-01. Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform.. https://doi.org/10.1364/ol.30.002266

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