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PubMed · 16200781

[Protective effect of human decay accelerating factor on mouse cells].

Y Zhao·L Li·Y Fu
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Abstract

OBJECTIVE: To observe the protective effect of human decay accelerating factor (DAF) expressed in mouse myeloma cells against the injury of human serum complement. METHODS: Mouse myeloma cells were transfected with human DAF cDNA and then put into normal human serum culture to observe if they were injured. RESULTS: The mouse cells containing human DAF cDNA and expressing human DAF survived for more than 48 hours and passed on from generation to generation in the culture medium containing human normal serum. However, the control mouse cells died in 1 - 4 hours. CONCLUSION: Human DAF protects mouse cells from injuring by human complement.

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AnimalsCD55 AntigensComplement System ProteinsDNA, ComplementaryGraft RejectionHumansMiceMultiple MyelomaProtective AgentsTransfectionTumor Cells, CulturedJournal Article

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Y Zhao, L Li, Y Fu. 2001-12-25. [Protective effect of human decay accelerating factor on mouse cells].. https://pubmed.ncbi.nlm.nih.gov/16200781/

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