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PubMed · 16212444

Impact of replicate types on proteomic expression analysis.

Natasha A Karp·Matthew Spencer·Helen Lindsay·Kevin O'Dell·Kathryn S Lilley
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Abstract

In expression proteomics, the samples utilized within an experimental design may include technical, biological, or pooled replicates. This manuscript discusses various experimental designs and the conclusions that can be drawn from them. Specifically, it addresses the impact of mixing replicate types on the statistical analysis which can be performed. This study focuses on difference gel electrophoresis (DiGE), but the issues are equally applicable to all quantitative methodologies assessing relative changes in protein expression.

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Analysis of VarianceAnimalsData Interpretation, StatisticalDrosophila melanogasterElectrophoresis, Gel, Two-DimensionalGene Expression ProfilingGene Expression RegulationIonsModels, StatisticalModels, TheoreticalMutation, MissenseOligonucleotide Array Sequence AnalysisProtein Array AnalysisProteomeProteomicsSoftwareJournal ArticleResearch Support, Non-U.S. Gov't

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Natasha A Karp, Matthew Spencer, Helen Lindsay, Kevin O'Dell, Kathryn S Lilley. Impact of replicate types on proteomic expression analysis.. https://doi.org/10.1021/pr050084g

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