Modular polyketide synthases: Investigating intermodular communication using 6 deoxyerythronolide B synthase module 2.

David A Moffet; Chaitan Khosla; David E Cane

doi:10.1016/j.bmcl.2005.09.017

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Modular polyketide synthases: Investigating intermodular communication using 6 deoxyerythronolide B synthase module 2.

David A Moffet·Chaitan Khosla·David E Cane

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Abstract

A novel variant of 6-deoxyerythronolide B synthase (DEBS) module 2 was constructed to explore the balance between protein-protein-mediated intermodular channeling and intrinsic substrate specificity within DEBS. This construct, termed (N3)Mod2+TE, was co-incubated with a complementary, donor form of the same module, (N5)Mod2(C2), as well as with a mutant of (N5)Mod2(C2) with an inactive ketosynthase domain, in order to determine the extent of intermediate channeling versus substrate diffusion into the downstream module.

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Chemistry, Pharmaceutical Drug Design Electrophoresis, Polyacrylamide Gel Lactones Models, Chemical Models, Molecular Multienzyme Complexes Polyketide Synthases Protein Binding Protein Structure, Tertiary Substrate Specificity Journal Article Research Support, N.I.H., Extramural

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David A Moffet, Chaitan Khosla, David E Cane. 2005-10-05. Modular polyketide synthases: Investigating intermodular communication using 6 deoxyerythronolide B synthase module 2.. https://doi.org/10.1016/j.bmcl.2005.09.017

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