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Detecting single stranded DNA with a solid state nanopore.

Daniel Fologea·Marc Gershow·Bradley Ledden·David S McNabb·Jene A Golovchenko·Jiali Li
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Abstract

Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than approximately 11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

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DNA, Single-StrandedElectrochemistryNanotechnologyNucleic Acid ConformationJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Daniel Fologea, Marc Gershow, Bradley Ledden, David S McNabb, Jene A Golovchenko, Jiali Li. 2005. Detecting single stranded DNA with a solid state nanopore.. https://doi.org/10.1021/nl051199m

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