Size sorting of protein assemblies using polymeric gradient surfaces.

Leonid Ionov; Manfred Stamm; Stefan Diez

doi:10.1021/nl051235h

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Size sorting of protein assemblies using polymeric gradient surfaces.

Leonid Ionov·Manfred Stamm·Stefan Diez

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Abstract

We report on a novel approach for the size-dependent fractionation of protein assemblies on polymeric surfaces. Using a simple temperature gradient method to generate one-dimensional gradients of grafted poly(ethylene glycol), we fabricated silicon-oxide chips with a gradually changing surface density of kinesin motor molecules. We demonstrate that such a bioactive surface can be used to sort gliding microtubules according to their length. To our knowledge, this is the first example of the self-organized sorting of protein assemblies on surfaces.

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Chemical Fractionation Kinesins Microchip Analytical Procedures Microtubule-Associated Proteins Microtubules Nanotechnology Oxides Silicon Temperature Journal Article Research Support, Non-U.S. Gov't

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Leonid Ionov, Manfred Stamm, Stefan Diez. 2005. Size sorting of protein assemblies using polymeric gradient surfaces.. https://doi.org/10.1021/nl051235h

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