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PubMed · 16220906

Novel fluorogenic substrates for imaging beta-lactamase gene expression.

Wenzhong Gao·Bengang Xing·Roger Y Tsien·Jianghong Rao
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Abstract

A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor.

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Cell LineFluorescent DyesGene ExpressionHumansHydrolysisLinear ModelsMolecular Structurebeta-LactamasesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Wenzhong Gao, Bengang Xing, Roger Y Tsien, Jianghong Rao. 2003-09-17. Novel fluorogenic substrates for imaging beta-lactamase gene expression.. https://doi.org/10.1021/ja036126o

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