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Development of a solid phase microextraction-gas chromatography method to determine N-hydroxymethyl-N-methylformamide and N-methylformamide in urine.

Vagner Fernandes Knupp·Edna Maria Alvarez Leite·Zenilda de Lourdes Cardeal
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Abstract

A headspace solid phase microextraction (SPME) method has been developed to determine metabolites of dimethylformamide, N-hydroxymethyl-N-methylformamide, and N-methylformamide (NMF) as NMF in urine by gas chromatography with nitrogen-phosphorus detector (GC-NPD). An SPME holder with a 65-microm PDMS/DVB fiber coating was used. Optimal desorption conditions were 250 degrees C for 1 min, adsorption at 80 degrees C for 15 min, and 3.00 mL of sample in the headspace vial. The method presented good resolution, repeatability, recovery, detection limit, ruggedness and response linearity.

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Chromatography, GasDimethylformamideFormamidesHumansReproducibility of ResultsUrineJournal ArticleResearch Support, Non-U.S. Gov't

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Vagner Fernandes Knupp, Edna Maria Alvarez Leite, Zenilda de Lourdes Cardeal. 2005-10-10. Development of a solid phase microextraction-gas chromatography method to determine N-hydroxymethyl-N-methylformamide and N-methylformamide in urine.. https://doi.org/10.1016/j.jchromb.2005.09.030

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