A biosensor in the time domain based on the diffusion coefficient measurement: intermolecular interaction of an intermediate of photoactive yellow protein.

Javaid Shahbaz Khan; Yasushi Imamoto; Yoichi Yamazaki; Mikio Kataoka; Fumio Tokunaga; Masahide Terazima

doi:10.1021/ac050559n

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A biosensor in the time domain based on the diffusion coefficient measurement: intermolecular interaction of an intermediate of photoactive yellow protein.

Javaid Shahbaz Khan·Yasushi Imamoto·Yoichi Yamazaki·Mikio Kataoka·Fumio Tokunaga·Masahide Terazima

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Abstract

A new type of biosensor is presented for the first time. This method is based on the diffusion measurement in the time domain by the transient grating method. As the first demonstration of this new method, various intermolecular interactions with a reaction intermediate of photoactive yellow protein, such as the protein-protein interaction, protein-DNA interaction, and protein-small molecule binding are detected. The characteristic advantages and limitations are summarized.

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Bacterial Proteins Biosensing Techniques DNA Diffusion Photoreceptors, Microbial Protein Binding Sensitivity and Specificity Time Factors Journal Article Research Support, Non-U.S. Gov't

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Javaid Shahbaz Khan, Yasushi Imamoto, Yoichi Yamazaki, Mikio Kataoka, Fumio Tokunaga, Masahide Terazima. 2005-10-15. A biosensor in the time domain based on the diffusion coefficient measurement: intermolecular interaction of an intermediate of photoactive yellow protein.. https://doi.org/10.1021/ac050559n

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