A novel microbial sensor using luminous bacteria.

S Lee; K Sode; K Nakanishi; J L Marty; E Tamiya; I Karube

doi:10.1016/0956-5663(92)87005-a

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A novel microbial sensor using luminous bacteria.

S Lee·K Sode·K Nakanishi·J L Marty·E Tamiya·I Karube

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Abstract

A novel microbial sensor system that uses luminous bacteria was developed for the determination of both glucose and toxic compounds. The sensor system consisted of a membrane with luminous bacteria immobilized upon it and a photomultiplier. Measurements were based on the in vivo intensity of the light emitted by the bacteria, as this is affected by their environment. A linear relationship was observed between increased luminescence and concentrations of glucose between 0.05 mM and 0.55 mM. The relative standard deviation was 10% for 0.55 mM glucose (n = 10). Toxic compounds such as benzalkonium chloride, sodium dodecyl sulphate and chromium(VI) were also detected by measuring the decrease in luminescence in their presence.

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Bacteria Benzalkonium Compounds Biosensing Techniques Chromium Glucose Luminescent Measurements Sodium Dodecyl Sulfate Journal Article

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S Lee, K Sode, K Nakanishi, J L Marty, E Tamiya, I Karube. 1992. A novel microbial sensor using luminous bacteria.. https://doi.org/10.1016/0956-5663(92)87005-a

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