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PubMed · 1623165

Function-blocking antibodies against cholinergic neuronal differentiation factor.

K Fukada·M F Towle
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Abstract

Cholinergic neuronal differentiation factor, CDF, causes a transition from noradrenergic to cholinergic phenotype in cultured sympathetic neurons. Moreover, its identification with leukemia inhibitory factor has shown that CDF is a multifunctional cytokine. To examine the physiological role of CDF and to further elucidate the as yet unknown effects of CDF on the nervous system, two kinds of function-blocking antibodies were generated. One type, raised against whole native CDF, completely blocks CDF activity, whereas the other type, raised against a synthetic peptide corresponding to the N-terminal amino acid region of CDF, blocks activity partially. All three anti-CDF and two antipeptide polyclonal antibodies tested in this study significantly inhibit CDF function.

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AnimalsAntibodiesAntibody SpecificityGrowth InhibitorsInterleukin-6Leukemia Inhibitory FactorLymphokinesNeuropeptidesRatsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

K Fukada, M F Towle. 1992. Function-blocking antibodies against cholinergic neuronal differentiation factor.. https://doi.org/10.1097/00001756-199202000-00009

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