Minimally invasive method for murine brain fixation.

Kenneth D Eichenbaum; Joseph W Eichenbaum; Ahmed Fadiel; Douglas C Miller; Necdet Demir; Frederick Naftolin; Arnold Stern; Paul H Pevsner

doi:10.2144/000112003

PubMed · 16235560

Minimally invasive method for murine brain fixation.

Kenneth D Eichenbaum·Joseph W Eichenbaum·Ahmed Fadiel·Douglas C Miller·Necdet Demir·Frederick Naftolin·Arnold Stern·Paul H Pevsner

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Abstract

Complete brain fixation can be achieved with transthoracic cardiac infusion without thoracotomy. Light and electron microscopy tissue sections reveal preservation of cytoplasmic and nuclear structure at all magnification levels. Punched samples were obtained from the fixed tissue specimens in precisely localized areas for study using electron microscopy. This perfusion fixation technique provides both faster tissue harvesting capability and higher quality tissue preservation, without the artifacts of brain swelling and ventricular dilation observed in direct cardiac perfusion. Acute, discrete change in brain tissue can be studied.

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Animals Brain Cytoplasm Fixatives Histological Techniques Mice Microscopy, Electron Microtomy Neurons Perfusion Preservation, Biological Tissue Embedding Tissue Preservation Journal Article

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Kenneth D Eichenbaum, Joseph W Eichenbaum, Ahmed Fadiel, Douglas C Miller, Necdet Demir, Frederick Naftolin, Arnold Stern, Paul H Pevsner. 2005. Minimally invasive method for murine brain fixation.. https://doi.org/10.2144/000112003

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