PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 16236236

New, rapid fluorescence stain method for histologic sections using lanthanide complexes.

Yunlan Su·Yizhuang Xu·Limin Yang·Jun Yang·Shifu Weng·Zhiwu Yu·Jinguang Wu
View original source ↗Export metadata ↓

Abstract

A new stain method for histologic sections different from traditional hematoxylin and eosin (H&E) methodology used two newly developed stain reagents from the lanthanide series, the antibiotic ofloxacin chelate of europium(III) and the ciprofloxacin hydrochloride chelate of terbium(III), exhibiting excellent fluorescence. These complexes are unique due to their nontoxicity, attractive fluorescent properties, rapid staining, and high water solubility. In this article, we focus on the interactions between biomolecules and synthesized lanthanide complexes and on the staining effect applied for tissue staining.

Explore related subjects

CiprofloxacinFluorescent DyesHumansKeratosisLanthanoid Series ElementsMicroscopy, FluorescenceOfloxacinPhotonsSalivary GlandsStaining and LabelingJournal ArticleResearch Support, Non-U.S. Gov't

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Yunlan Su, Yizhuang Xu, Limin Yang, Jun Yang, Shifu Weng, Zhiwu Yu, Jinguang Wu. 2005-09-28. New, rapid fluorescence stain method for histologic sections using lanthanide complexes.. https://doi.org/10.1016/j.ab.2005.09.010

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2026

α-Ketoglutarate Promotes Ciprofloxacin Tolerance in Methicillin-Resistant Staphylococcus aureus via Glutamate-Mediated Metabolic Remodeling.

Methicillin-resistant Staphylococcus aureus (MRSA) is a globally significant pathogen causing severe infections. The chronicity and recurrence of its infection pose serious challenges to public health. In this study, we reported that intracellular accumulation of α-ketoglutarate (α-KG) significantly increased ciprofloxacin (CIP) tolerance in MRSA. Using integrated metabolomic and functional genomic approaches, we demonstrated that both exogenous α-KG and genetic knockout of α-KG dehydrogenase (ΔsucA, ΔsucB) induced CIP tolerance in MRSA. Mechanistically, elevated α-KG levels drive the accumulation of glutamate (Glu), which in turn reduced bacterial membrane potential and cellular ATP content. Furthermore, Glu accumulation raised intracellular osmotic pressure, leading to decreased CIP uptake. These metabolic alterations enable MRSA to sustain high tolerance toward ciprofloxacin. Our findings reveal a key role of the α-KG-Glu metabolic axis in driving antibiotic tolerance and provide novel insights into the metabolic adaptations underlying drug persistence in MRSA.

Ciprofloxacin↗
PubMed2006

Partitioning and photodegradation of ciprofloxacin in aqueous systems in the presence of organic matter.

Ciprofloxacin is an extensively used antibiotic that has been reported to occur in surface water. Previous studies have indicated that ciprofloxacin photodegrades and sorbs to particulate organic material within aquatic systems. The first objective of the current study was to evaluate the influence of organic material on photodegradation rates of ciprofloxacin. Using a bench top experimental design, ciprofloxacin was added to experimental chambers that contained only water or water and fine particulate organic matter (FPOM) followed by exposure to ultraviolet light. Sorption to FPOM was rapid, reducing the amount of ciprofloxacin that was available for photodegradation. Thus, the presence of FPOM initially decreased the ciprofloxacin concentration in the aqueous compartment. However by the end of the 16 h test, 42% of the ciprofloxacin was recovered from the test system with FPOM present, while only 2% of the ciprofloxacin was recovered in systems that did not contain FPOM. The second objective of this study was to compare the sorption coefficients for ciprofloxacin between two types of organic material: FPOM, classified as amphipod processed leaves, and coarse particulate organic matter (CPOM), represented by intact leaf disks. Sorption to FPOM (log Kd of 4.54+/-0.09 l kg(-1)) was 1.6 orders of magnitude greater than sorption to CPOM (log Kd of 2.92+/-0.10 l kg(-1)) potentially resulting in differential toxicity among similar organisms that occupy these different niches and leading to different estimates of environmental fate and effects.

Ciprofloxacin↗