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PubMed · 16248057

A solid supported reagent for internucleoside H-phosphonate linkage formation.

Nikhil Mohe·Petri Heinonen·Yogesh S Sanghvi·Roger Strömberg
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Abstract

A fast and convenient procedure for synthesis of dinucleoside H-phosphonates is obtained through use of the novel polystyrene supported 5-carboxy-5-methyl-2-oxo-2-chloro-1,3,2-diaoxaphosphorinane reagent. Virtually quantitative H-phosphonate condensations are obtained leading to excellent isolatedyields and with only a simple filtration as the purification procedure. This provides for a convenient and high-yielding procedure that should be suited for solution-phase synthesis of oligonucleotides.

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Indicators and ReagentsMagnetic Resonance SpectroscopyModels, ChemicalNucleosidesOligodeoxyribonucleotidesOligonucleotidesOrganophosphatesOrganophosphonatesOxidantsSpectrometry, Mass, Electrospray IonizationJournal Article

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BibTeXRIS

Nikhil Mohe, Petri Heinonen, Yogesh S Sanghvi, Roger Strömberg. 2005. A solid supported reagent for internucleoside H-phosphonate linkage formation.. https://doi.org/10.1081/ncn-200059257

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