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PubMed · 16251183

Galpha12/13 is essential for directed cell migration and localized Rho-Dia1 function.

Polyxeni Goulimari·Thomas M Kitzing·Helga Knieling·Dominique T Brandt·Stefan Offermanns·Robert Grosse
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Abstract

Scratch-wound assays are frequently used to study directed cell migration, a process critical for embryogenesis, invasion, and tissue repair. The function and identity of trimeric G-proteins in cell behavior during wound healing is not known. Here we show that Galpha12/13, but not Galphaq/11 or Galphai, is indispensable for coordinated and directed cell migration. In mouse embryonic fibroblasts endogenous Rho activity is present at the rear of migrating cells but also at the leading edge, whereas it is undetectable at the cell front of Galpha12/13-deficient mouse embryonic fibroblasts. Spatial activation of Rho at the wound edge can be stimulated by lysophosphatidic acid. Active Rho colocalizes with the diaphanous-related formin Dia1 at the cell front. Galpha12/13-deficient cells lack Dia1 localization to the wound edge and are unable to form orientated, stable microtubules during wound healing. Knock down of Dia1 reveals its requirement for microtubule stabilization as well as polarized cell migration. Thus, we identified Galpha12/13-proteins as essential components linking extracellular signals to localized Rho-Dia1 function during directed cell movement.

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Affinity LabelsAnimalsCarrier ProteinsCell LineCell MovementCytoskeletonFluorescent Antibody TechniqueForminsGTP-Binding Protein alpha Subunits, G12-G13MiceMicrotubulesRNA InterferenceRecombinant Proteinsrho GTP-Binding ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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Polyxeni Goulimari, Thomas M Kitzing, Helga Knieling, Dominique T Brandt, Stefan Offermanns, Robert Grosse. 2005-10-25. Galpha12/13 is essential for directed cell migration and localized Rho-Dia1 function.. https://doi.org/10.1074/jbc.m508690200

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