Retroviral restriction factor TRIM5alpha is a trimer.

Claudia C Mische; Hassan Javanbakht; Byeongwoon Song; Felipe Diaz-Griffero; Matthew Stremlau; Bettina Strack; Zhihai Si; Joseph Sodroski

doi:10.1128/jvi.79.22.14446-14450.2005

PubMed · 16254380

Retroviral restriction factor TRIM5alpha is a trimer.

Claudia C Mische·Hassan Javanbakht·Byeongwoon Song·Felipe Diaz-Griffero·Matthew Stremlau·Bettina Strack·Zhihai Si·Joseph Sodroski

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Abstract

The retrovirus restriction factor TRIM5alpha targets the viral capsid soon after entry. Here we show that the TRIM5alpha protein oligomerizes into trimers. The TRIM5alpha coiled-coil and B30.2(SPRY) domains make important contributions to the formation and/or stability of the trimers. A functionally defective TRIM5alpha mutant with the RING and B-box 2 domains deleted can form heterotrimers with wild-type TRIM5alpha, accounting for the observed dominant-negative activity of the mutant protein. Trimerization potentially allows TRIM5alpha to interact with threefold pseudosymmetrical structures on retroviral capsids.

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Animals Binding Sites Capsid Capsid Proteins Cell Line Chlorocebus aethiops Genetic Variation HeLa Cells Humans Macaca mulatta Proteins Retroviridae Transfection Ubiquitin-Protein Ligases Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Claudia C Mische, Hassan Javanbakht, Byeongwoon Song, Felipe Diaz-Griffero, Matthew Stremlau, Bettina Strack, Zhihai Si, Joseph Sodroski. 2005. Retroviral restriction factor TRIM5alpha is a trimer.. https://doi.org/10.1128/jvi.79.22.14446-14450.2005

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