Ordered differential display.

Mikhail V Matz; Ella A Meleshkevitch

doi:10.1385/1-59259-968-0:059

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Ordered differential display.

Mikhail V Matz·Ella A Meleshkevitch

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Abstract

Ordered differential display (ODD) is one of the approaches that uses systematic, rather than random, sampling of transcripts for display and thereby provides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material. The protocol outlined here, in addition to ODD procedure itself, also describes isolation of RNA and synthesis of double-stranded cDNA from small biological samples.

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Base Sequence DNA Primers DNA, Complementary Electrophoresis Gene Expression Profiling Genetic Techniques Models, Genetic Molecular Sequence Data Oligonucleotides Polymerase Chain Reaction RNA RNA, Messenger Reproducibility of Results Journal Article

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Mikhail V Matz, Ella A Meleshkevitch. 2006. Ordered differential display.. https://doi.org/10.1385/1-59259-968-0%3A059

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