PLP-1 binds nematode double-stranded telomeric DNA.

Seol Hee Im; Junho Lee

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PubMed · 16267406

PLP-1 binds nematode double-stranded telomeric DNA.

Seol Hee Im·Junho Lee

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Abstract

The integrity and proper functioning of telomeres require association of telomeric DNA sequences with specific binding proteins. We have characterized PLP-1, a PURa homolog encoded by F45E4.2, which we previously identified as a candidate double stranded telomere binding protein, by affinity chromatography followed by mass spectrometry. PLP-1 bound double-stranded telomeric DNA in vitro as shown by competition assays. Core binding was provided by the third and fourth nucleotides of the TTAGGC telomeric repeat. This is quite different from the binding sequence of CEH-37, another C. elegans telomere binding protein, suggesting that multiple proteins may bind nematode telomeric DNA simultaneously in vivo.

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Animals Base Sequence Binding Sites Caenorhabditis elegans Caenorhabditis elegans Proteins Carrier Proteins Electrophoretic Mobility Shift Assay Models, Molecular Molecular Sequence Data Telomere Telomere-Binding Proteins Journal Article Research Support, Non-U.S. Gov't

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Seol Hee Im, Junho Lee. 2005-10-31. PLP-1 binds nematode double-stranded telomeric DNA.. https://pubmed.ncbi.nlm.nih.gov/16267406/

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