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PubMed · 16278842

A novel processing method for injection-molded polyether-urethane scaffolds. Part 2: cellular interactions.

H Haugen·J Aigner·M Brunner·E Wintermantel
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Abstract

A large-scale scaffold processing method with injection molding has been successfully developed. Water was used as a foaming agent for the new technique. NaCl was used as a porogen to achieve an open-cell structure. Organic solvents, which are common foaming agents for polyurethane, where not used. Toxic remains in the polymer were therefore prevented. Biocompatibility tested gave a mean optical density of 81% from WST-1 proliferation assay. In comparison to the previously study processing method, hot pressing (Haugen H, Ried V, Brunner M, Will J, Wintermantel E. J Mater Sci: Mater Med2004;15:343-346), the current scaffolds had an increase of 20% of the mean optical density. Cell seeding showed that human fibroblasts adhered to the surface and proliferated. The spread of the adhered fibroblasts was uniform on the surface. A quantitative MTT analysis proved that there was a significant (p < 0.01) increase in the OD level after 7 and 14 days of incubation. This cell layer thickened with increased incubation time from 7 to 14 days (p < 0.05) and had typical fibroblast morphology.

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Biocompatible MaterialsBiomedical TechnologyCell SurvivalCells, CulturedFibroblastsHumansMaterials TestingMercuryPolyurethanesPorositySodium ChlorideStress, MechanicalTensile StrengthWaterEvaluation StudyJournal Article

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BibTeXRIS

H Haugen, J Aigner, M Brunner, E Wintermantel. 2006. A novel processing method for injection-molded polyether-urethane scaffolds. Part 2: cellular interactions.. https://doi.org/10.1002/jbm.b.30397

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