Liquid-based hybridization assay with real-time detection in miniaturized array platforms.

Anna Guerasimova; Lajos Nyarsik; Jian-Ping Liu; Regine Schwartz; Matthias Lange; Hans Lehrach; Michal Janitz

doi:10.1016/j.bioeng.2005.09.005

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Liquid-based hybridization assay with real-time detection in miniaturized array platforms.

Anna Guerasimova·Lajos Nyarsik·Jian-Ping Liu·Regine Schwartz·Matthias Lange·Hans Lehrach·Michal Janitz

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Abstract

An assay for the fluorescent detection of short oligonucleotide probe hybridization in miniaturized high-density array platforms is presented. It combines hybridization in solution with real-time fluorescent detection, which involves measurement of fluorescence increase by means of an induced fluorescence resonance energy transfer. The feasibility of this approach using DNA or RNA as a target, and short DNA- as well as LNA (locked nucleic acid)-modified oligonucleotides as probes is shown. The presented approach could potentially contribute to a significant increase in the throughput of large-scale genomic applications, such as oligofingerprinting and genotyping, and also reduce material consumption.

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Computer Systems DNA Fingerprinting Equipment Design Equipment Failure Analysis Feasibility Studies Fluorescence Resonance Energy Transfer In Situ Hybridization, Fluorescence Microfluidic Analytical Techniques Miniaturization Oligonucleotide Array Sequence Analysis Reproducibility of Results Sensitivity and Specificity Solutions Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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Anna Guerasimova, Lajos Nyarsik, Jian-Ping Liu, Regine Schwartz, Matthias Lange, Hans Lehrach, Michal Janitz. 2005-11-18. Liquid-based hybridization assay with real-time detection in miniaturized array platforms.. https://doi.org/10.1016/j.bioeng.2005.09.005

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