Global synthetic-lethality analysis and yeast functional profiling.

Siew Loon Ooi; Xuewen Pan; Brian D Peyser; Ping Ye; Pamela B Meluh; Daniel S Yuan; Rafael A Irizarry; Joel S Bader; Forrest A Spencer; Jef D Boeke

doi:10.1016/j.tig.2005.11.003

PubMed · 16309778

Global synthetic-lethality analysis and yeast functional profiling.

Siew Loon Ooi·Xuewen Pan·Brian D Peyser·Ping Ye·Pamela B Meluh·Daniel S Yuan·Rafael A Irizarry·Joel S Bader·Forrest A Spencer·Jef D Boeke

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Abstract

The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.

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Chromosome Mapping Gene Deletion Gene Expression Profiling Genes, Fungal Genes, Lethal Genetic Techniques Genome, Fungal Oligonucleotide Array Sequence Analysis RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Journal Article Research Support, N.I.H., Extramural Review

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Siew Loon Ooi, Xuewen Pan, Brian D Peyser, Ping Ye, Pamela B Meluh, Daniel S Yuan, Rafael A Irizarry, Joel S Bader, Forrest A Spencer, Jef D Boeke. 2005-11-23. Global synthetic-lethality analysis and yeast functional profiling.. https://doi.org/10.1016/j.tig.2005.11.003

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