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Edge plane pyrolytic graphite electrodes in electroanalysis: an overview.

Craig E Banks·Richard G Compton
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Abstract

The recent development, behavior and scope of edge plane pyrolytic graphite electrodes in electroanalysis are overviewed. Similarities to, and advantages, over multi-walled CNT modified electrodes are noted and the wide scope of applications, ranging through gas sensing, stripping voltammetry and biosensing, illustrated.

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Ascorbic AcidCarbonChemistry Techniques, AnalyticalElectrochemistryElectrodesGasesGraphiteHalogensNADNanotubes, CarbonOxidation-ReductionSulfhydryl CompoundsJournal ArticleReview

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Craig E Banks, Richard G Compton. 2005. Edge plane pyrolytic graphite electrodes in electroanalysis: an overview.. https://doi.org/10.2116/analsci.21.1263

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Ascorbic acid (AsA) is an essential nutritional component and powerful antioxidant in vegetables, and in plants, AsA levels are regulated by light. AsA levels in the leaves of Chinese chive (Allium tuberosum Rottler ex Spr), a popular vegetable, are poorly understood. Thus, this study was performed to assess the influence of light on AsA biosynthesis in chive and select related genes (AtuGGP1 and AtuGME1); in addition, bioinformatic analyses and gene expression level assays were performed. The biological information obtained for AtuGGP1 and AtuGME1 was analysed with several tools, including NCBI, DNAMAN, and MEGA11. After different light treatments were performed, the Chive AsA content and AtuGGP1 and AtuGME1 expression levels were determined. These results suggest that 1) compared with natural light, continuous darkness inhibited AsA synthesis in chives. 2) The amino acid sequences of AtuGGP1 and AtuGME1 are very similar to those of other plants. 3) The trends observed for the expression levels of AtuGGP1 and AtuGME1 were consistent with the AsA content observed in chives. Hence, we speculated that light controls AsA biosynthesis in chives by regulating AtuGGP1 and AtuGME1 expression. This study provided impactful and informative evidence regarding the functions of GGP and GME in chives.

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