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Pyrosequencing Bacillus anthracis.

Tara Wahab·Sandra Hjalmarsson·Ralfh Wollin·Lars Engstrand
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Abstract

Pyrosequencing technology is a sequencing method that screens DNA nucleotide incorporation in real time. A set of coupled enzymatic reactions, together with bioluminescence, detects incorporated nucleotides in the form of light pulses, which produces a profile of characteristic peaks in a pyrogram. We used this technology to identify the warfare agent Bacillus anthracis by sequencing 4 single nucleotide polymorphisms (SNPs) in the rpoB gene as chromosomal markers for B. anthracis. In addition, 1 segment in each of the B. anthracis plasmids pXO1 and pXO2 was analyzed to determine the virulence status of the bacterial strains. Pyrosequencing technology is a powerful method to identify B. anthracis.

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AnimalsBacillusBacillus anthracisBacterial Typing TechniquesDNA-Directed RNA PolymerasesGenotypeHumansPlasmidsPolymerase Chain ReactionPolymorphism, Single NucleotideSequence Analysis, DNASpecies SpecificityVirulenceEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Tara Wahab, Sandra Hjalmarsson, Ralfh Wollin, Lars Engstrand. 2005. Pyrosequencing Bacillus anthracis.. https://doi.org/10.3201/eid1110.041316

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