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PubMed · 16329844

[Reconstruction of dentin-pulp complex structure by tissue engineering technology].

Hong-yan Guo·Bu-ling Wu·Xi-min Guo·Cheng Yang·Peng Xu·Chang-yong Wang
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Abstract

OBJECTIVE: To investigate the possibility of reconstruction of dentin-pulp complex by tissue engineering technology. METHODS: Rat dental pulp stem cells were seeded into HA-TCP scaffold and incubated for 20 hours in vitro. Then the cell-scaffold complex was implanted subcutaneously into the dorsal side of nude mice. 8 weeks postimplantation, the samples were extracted for histological and immunohistochemical examinations. RESULTS: Three strata of tissue were observed in the hole of HA-TCP scaffold. They were dentin-like tissue, predentin-like tissue and pulp-like tissue respectively from the inner surface of the pore to the center. Dentin tubules were obvious in predentin-like and dentin-like tissue lining from the pulp-like tissue through predentin-like tissue and dentin-like tissue. Cells localized along the edge of pulp-like tissue were dense and polarized, resembling odontoblasts. Immunohistochemical study demonstrated DSP and DMP1 expression in these odontoblast-like cells and in the area of predentin-like tissue. CONCLUSIONS: Tissue-engineered rat dentin-pulp complex was reconstructed by seeding HA-TCP scaffold with rat dental pulp stem cells.

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AnimalsCalcium PhosphatesCells, CulturedDental PulpDentinFemaleHydroxyapatitesMiceMice, Inbred BALB CMice, NudeRatsStem CellsTissue EngineeringTissue ScaffoldsEnglish AbstractJournal Article

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Hong-yan Guo, Bu-ling Wu, Xi-min Guo, Cheng Yang, Peng Xu, Chang-yong Wang. 2005. [Reconstruction of dentin-pulp complex structure by tissue engineering technology].. https://pubmed.ncbi.nlm.nih.gov/16329844/

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