Proteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization.

Laurent Miguet; Karine Pacaud; Claire Felden; Bénédicte Hugel; M Carmen Martinez; Jean-Marie Freyssinet; Raoul Herbrecht; Noelle Potier; Alain van Dorsselaer; Laurent Mauvieux

doi:10.1002/pmic.200500133

PubMed · 16342139

Proteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization.

Laurent Miguet·Karine Pacaud·Claire Felden·Bénédicte Hugel·M Carmen Martinez·Jean-Marie Freyssinet·Raoul Herbrecht·Noelle Potier·Alain van Dorsselaer·Laurent Mauvieux

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Abstract

Shed membrane microparticles (MPs) are microvesicles generated from the plasma membrane when cells are submitted to stress conditions. Although MPs reflect the cell state (at least in vitro), little is known on their protein composition. We describe the first set of experiments aiming to characterize the MP proteome. Two ways of triggering MP formation from a T-lymphocytic cell line were analyzed using a 1-D gel approach coupled with LC-MS/MS and the results were compared with those obtained from a classic membrane preparation. In total, 390 proteins were identified in MPs, among which 34% were localized to the plasma membrane. The MPs revealed a broad representation of plasma membrane proteins including 17 hematopoietic clusters of differentiation. This approach was successfully applied to one human chronic B-cell lymphoid malignancy. In all, 413 proteins were identified, including 117 membrane proteins, many of them being pathology associated. The sequence coverage in identified proteins was improved combining both nano-LC-MS/MS and MALDI-MS data. The suppression effect, observed on very complex peptide mixtures, was remediated by chromatographic fractionation. MPs may represent a new tool for studying plasma membrane proteins, displaying the advantages of reproducibility, minimal organelle contamination, and being potentially applicable to most cell types.

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Cell Membrane Chromatography, Liquid Electrophoresis, Polyacrylamide Gel Humans Lymphocytes Lymphoma, B-Cell Nanotechnology Proteome Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tumor Cells, Cultured Journal Article

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Laurent Miguet, Karine Pacaud, Claire Felden, Bénédicte Hugel, M Carmen Martinez, Jean-Marie Freyssinet, Raoul Herbrecht, Noelle Potier, Alain van Dorsselaer, Laurent Mauvieux. 2006. Proteomic analysis of malignant lymphocyte membrane microparticles using double ionization coverage optimization.. https://doi.org/10.1002/pmic.200500133

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