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A low-cost open-source SNP genotyping platform for association mapping applications.

Stuart J Macdonald·Tomi Pastinen·Anne Genissel·Theodore W Cornforth·Anthony D Long
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Abstract

Association mapping aimed at identifying DNA polymorphisms that contribute to variation in complex traits entails genotyping a large number of single-nucleotide polymorphisms (SNPs) in a very large panel of individuals. Few technologies, however, provide inexpensive high-throughput genotyping. Here, we present an efficient approach developed specifically for genotyping large fixed panels of diploid individuals. The cost-effective, open-source nature of our methodology may make it particularly attractive to those working in nonmodel systems.

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AnimalsChromosome MappingCost-Benefit AnalysisDrosophila melanogasterGenome, InsectGenotypeOligonucleotidesPolymorphism, Single NucleotideSensitivity and SpecificityJournal ArticleResearch Support, N.I.H., Extramural

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Stuart J Macdonald, Tomi Pastinen, Anne Genissel, Theodore W Cornforth, Anthony D Long. 2005-12-02. A low-cost open-source SNP genotyping platform for association mapping applications.. https://doi.org/10.1186/gb-2005-6-12-r105

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