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Estimating perfusion using microCT to locate microspheres.

M Marxen·C Paget·L X Yu·R M Henkelman
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Abstract

The injection of microspheres into the blood stream has been a common method to measure the spatial distribution of blood flow (perfusion). A technique to conduct this kind of measurement in small animal organs is presented using silver-coated microspheres with a diameter of 16 microm and high-resolution computed tomography (microCT) to detect individual microspheres. Phantom experiments demonstrate the detectability of individual spheres. The distribution of microspheres within a rat heart is given as an example. Using non-destructive, three-dimensional imaging for microsphere detection avoids the cumbersome dissection of the organ into samples or slices and their subsequent registration. The detection of individual spheres allows high-resolution measurements of perfusion and arbitrary definition of regions of interest. These, in turn, allow for accurate statistical analysis of perfusion such as relative dispersion curves.

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AnimalsCoronary CirculationMicroscopy, Electron, ScanningMicrospheresModels, CardiovascularModels, StatisticalMyocardiumPerfusionPhantoms, ImagingRatsRegional Blood FlowTomography, X-Ray ComputedJournal ArticleResearch Support, Non-U.S. Gov't

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M Marxen, C Paget, L X Yu, R M Henkelman. 2005-12-15. Estimating perfusion using microCT to locate microspheres.. https://doi.org/10.1088/0031-9155%2F51%2F1%2Fn02

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