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A single mode fibre-optic evanescent wave biosensor.

E E Carlyon·C R Lowe·D Reid·I Bennion
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Abstract

This paper reports experimental developments in the construction and operation of a single-mode fibre-optic evanescent wave biosensor using an exposed core silica single-mode fibre embedded in a silica block. The device was able to monitor the concentration of a blue dye, Procion Blue MX-G, in overlayers of various refractive indices. The practicality of such a biosensor has been demonstrated with a colorimetric enzyme assay system. Penicillin G in the 0-0.4 mM concentration range was monitored at 633 nm by the decoloration of the starch-iodine reagent when Bacillus cereus penicillinase was immobilized over the exposed core of the monomode fibre.

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Biosensing TechniquesColorimetryColoring AgentsFiber Optic TechnologyPenicillin GSpectrophotometryJournal ArticleResearch Support, Non-U.S. Gov't

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E E Carlyon, C R Lowe, D Reid, I Bennion. 1992. A single mode fibre-optic evanescent wave biosensor.. https://doi.org/10.1016/0956-5663(92)90019-j

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