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PubMed · 1637864

Ribosome function determined by fluorescence.

B Hardesty·O W Odom·W Picking
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Abstract

Five different fluorescence phenomena are considered in relation to their use to study the structure and function of ribosomes. These are: quantum yield or emission intensity; emission wavelength maximum; fluorescence anisotropy; collisional quenching; and nonradiative energy transfer. Results from a number of studies in which these techniques were used are described and summarized in relation to the movement and conformation of tRNA, the nascent peptide, and mRNA in a ribosome during the reaction steps of peptide elongation.

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Base SequenceFluorescenceMolecular Sequence DataNucleic Acid ConformationRNA, MessengerRNA, TransferRibosomesSpectrometry, FluorescenceJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Review

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B Hardesty, O W Odom, W Picking. 1992. Ribosome function determined by fluorescence.. https://doi.org/10.1016/0300-9084(92)90117-w

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