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Coreceptor usage of primary HIV type 1 isolates obtained from different lymph node subsets.

Charlotte Casper·Mattias Mild·Marianne Jansson·Anders Karlsson·Viveca Holmberg·Göran Bratt·Hubertus Van Paaschen·Peter Biberfeld·Asa Björndal·Jan Albert·Mikulas Popovic·Eva Maria Fenyö
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Abstract

Biological characteristics of virus quantitatively rescued from different cell types present in lymph nodes of HIV-1-infected individuals in various stages of their disease were determined, not including patients with AIDS defining illness. Viruses were obtained by cocultivation with donor monocyte-derived macrophages and T-lymphocytes and their biological phenotype compared to viruses obtained from the peripheral blood mononuclear cells of the same patient. The biological phenotype was determined on established cell lines (U937-2, CEM, and MT-2) and on the U87.CD4 coreceptor indicator cell lines and variable region 3 (V3) of the envelope was subjected to direct sequencing. All isolates obtained from lymph node subsets used CCR5 as coreceptor. Furthermore, these viruses were also sensitive to inhibition by beta-chemokines as analyzed for viruses of one patient. All 12 V3 regions showed a unique sequence indicating compartmentalization within each patient. The biological phenotype of CCR5-dependent (R5) HIV-1 isolates obtained from PBMC resembles the phenotype of viruses isolated from different lymph node cell subsets.

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Amino Acid SequenceChemokines, CCHIV InfectionsHIV-1HumansLeukocytes, MononuclearLymph NodesMolecular Sequence DataPhenotypeReceptors, CCR5Virus ReplicationJournal ArticleResearch Support, Non-U.S. Gov't

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Charlotte Casper, Mattias Mild, Marianne Jansson, Anders Karlsson, Viveca Holmberg, Göran Bratt, Hubertus Van Paaschen, Peter Biberfeld, Asa Björndal, Jan Albert, Mikulas Popovic, Eva Maria Fenyö. 2005. Coreceptor usage of primary HIV type 1 isolates obtained from different lymph node subsets.. https://doi.org/10.1089/aid.2005.21.1003

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