Ewald sphere correction for single-particle electron microscopy.

Matthias Wolf; David J DeRosier; Nikolaus Grigorieff

doi:10.1016/j.ultramic.2005.11.001

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Ewald sphere correction for single-particle electron microscopy.

Matthias Wolf·David J DeRosier·Nikolaus Grigorieff

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Abstract

Most algorithms for three-dimensional (3D) reconstruction from electron micrographs assume that images correspond to projections of the 3D structure. This approximation limits the attainable resolution of the reconstruction when the dimensions of the structure exceed the depth of field of the microscope. We have developed two methods to calculate a reconstruction that corrects for the depth of field. Either method applied to synthetic data representing a large virus yields a higher resolution reconstruction than a method lacking this correction.

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Algorithms Data Display Fourier Analysis Imaging, Three-Dimensional Microscopy, Electron Viruses Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

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Matthias Wolf, David J DeRosier, Nikolaus Grigorieff. 2005-12-09. Ewald sphere correction for single-particle electron microscopy.. https://doi.org/10.1016/j.ultramic.2005.11.001

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