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PubMed · 16390984

Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity.

Robin Warren·Marianna de Kock·Erica Engelke·Roseline Myburgh·Nico Gey van Pittius·Thomas Victor·Paul van Helden
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Abstract

Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist.

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Bacteriological TechniquesDNA, BacterialHot TemperatureHumansLaboratory InfectionMycobacterium InfectionsMycobacterium tuberculosisSafety ManagementJournal Article

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Robin Warren, Marianna de Kock, Erica Engelke, Roseline Myburgh, Nico Gey van Pittius, Thomas Victor, Paul van Helden. 2006. Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity.. https://doi.org/10.1128/jcm.44.1.254-256.2006

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