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A simple and extremely sensitive system for detecting estrogenic activity using transgenic Arabidopsis thaliana.

Takuto Tojo·Kenichi Tsuda·Tomoko S Wada·Ken-ichi Yamazaki
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Abstract

Numerous physiological processes are regulated by endocrine systems in animals. Endocrine-disrupting chemicals (EDCs) can affect physiological processes of organisms by binding to hormone receptors. Therefore, it is necessary to develop methods for detecting EDCs and removing them from the environment. We have developed a simple and low-cost reporter gene assay system for the comprehensive analysis of estrogenic activity using transgenic Arabidopsis thaliana. This transgenic plant constantly expresses two effector proteins: a chimeric estrogen receptor and a chimeric nuclear receptor coactivator. Estrogen-dependent interaction between the two effector proteins triggers transcriptional activation of reporter gene, beta-glucuronidase. We have demonstrated this transgenic plant's capability of detecting the existence of 17beta-estradiol at a concentration of 50 pM (13 pg/ml) in agar medium. This plant can also detect other estrogenic substances, such as diethylstilbestrol, p-n-nonylphenol, bisphenol A, and Genistein.

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AnimalsArabidopsisBenzhydryl CompoundsBiological AssayEndocrine DisruptorsEnvironmental MonitoringEstradiolEstrogens, Non-SteroidalGenes, ReporterGenisteinGlucuronidaseNuclear Receptor Coactivator 2PhenolsPlants, Genetically ModifiedReceptors, EstrogenSensitivity and SpecificityTranscriptional ActivationBisphenol A CompoundsEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Takuto Tojo, Kenichi Tsuda, Tomoko S Wada, Ken-ichi Yamazaki. 2006. A simple and extremely sensitive system for detecting estrogenic activity using transgenic Arabidopsis thaliana.. https://doi.org/10.1016/j.ecoenv.2005.03.014

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