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Two-dimensional mass spectrometric mapping.

Christoph Roesli·Giuliano Elia·Dario Neri
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Abstract

The identification and relative quantification of proteins in closely related biological samples is the backbone for many investigations in systems biology and for the discovery of biomarkers. While two-dimensional gel-based methodologies are still widely used for comparative proteomic studies, the recent advent of gel-free methodologies may allow the analysis of a larger number of samples in an automated fashion. Most of the technologies presented in this review require a chemical modification of proteins before analysis, and rely on the relative intensities of mass spectrometry signals for protein quantification. In particular, two-dimensional mass spectrometric mapping methodologies provide a visual representation of mass spectrometric data, thus facilitating the identification of differences in relative protein abundance.

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HumansMass SpectrometryPeptidesProteinsProteomicsSoftwareJournal ArticleResearch Support, Non-U.S. Gov'tReview

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Christoph Roesli, Giuliano Elia, Dario Neri. 2006-01-06. Two-dimensional mass spectrometric mapping.. https://doi.org/10.1016/j.cbpa.2005.12.017

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