Lipid profiling by multiple precursor and neutral loss scanning driven by the data-dependent acquisition.

Dominik Schwudke; Jeffrey Oegema; Lyle Burton; Eugeni Entchev; J Thomas Hannich; Christer S Ejsing; Teymuras Kurzchalia; Andrej Shevchenko

doi:10.1021/ac051605m

PubMed · 16408944

Lipid profiling by multiple precursor and neutral loss scanning driven by the data-dependent acquisition.

Dominik Schwudke·Jeffrey Oegema·Lyle Burton·Eugeni Entchev·J Thomas Hannich·Christer S Ejsing·Teymuras Kurzchalia·Andrej Shevchenko

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Abstract

Data-dependent acquisition of MS/MS spectra from lipid precursors enables to emulate the simultaneous acquisition of an unlimited number of precursor and neutral loss scans in a single analysis. This approach takes full advantage of rich fragment patterns in tandem mass spectra of lipids and enables their profiling by complex (Boolean) scans, in which masses of several fragment ions are considered within a single logical framework. No separation of lipids is required, and the accuracy of identification and quantification is not compromised, compared to conventional precursor and neutral loss scanning.

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Animals Caenorhabditis elegans Chromatography, Liquid Lipids Tandem Mass Spectrometry Journal Article Research Support, Non-U.S. Gov't

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Dominik Schwudke, Jeffrey Oegema, Lyle Burton, Eugeni Entchev, J Thomas Hannich, Christer S Ejsing, Teymuras Kurzchalia, Andrej Shevchenko. 2006-01-15. Lipid profiling by multiple precursor and neutral loss scanning driven by the data-dependent acquisition.. https://doi.org/10.1021/ac051605m

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