Erythrocyte structure and dynamics quantified by Hilbert phase microscopy.

Gabriel Popescu; Takahiro Ikeda; Catherine A Best; Kamran Badizadegan; Ramachandra R Dasari; Michael S Feld

doi:10.1117/1.2149847

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Erythrocyte structure and dynamics quantified by Hilbert phase microscopy.

Gabriel Popescu·Takahiro Ikeda·Catherine A Best·Kamran Badizadegan·Ramachandra R Dasari·Michael S Feld

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Abstract

We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.

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Cell Size Cells, Cultured Equipment Design Equipment Failure Analysis Erythrocytes Hemolysis Humans Image Enhancement Image Interpretation, Computer-Assisted Imaging, Three-Dimensional Microscopy, Phase-Contrast Evaluation Study Letter Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

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Gabriel Popescu, Takahiro Ikeda, Catherine A Best, Kamran Badizadegan, Ramachandra R Dasari, Michael S Feld. Erythrocyte structure and dynamics quantified by Hilbert phase microscopy.. https://doi.org/10.1117/1.2149847

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