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Frame shuffling: a novel method for in vitro protein evolution.

Kenji Kashiwagi·Yasuhiro Isogai·Kei-ichi Nishiguchi·Kiyotaka Shiba
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Abstract

We describe 'frame shuffling', a novel method for preparing artificial protein libraries. With this method, a Y-family DNA polymerase known to introduce frame shift mutations at high rates is utilized to scramble the reading frames of a parental gene. The resultant progeny produce mutant proteins having segmental sequence changes. Such frame-shuffled mutant proteins exhibit physicochemical properties that differ from those of proteins obtained using conventional mutagenesis.

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Amino Acid SequenceCentrifugation, Density GradientCircular DichroismCodon, TerminatorDNA Polymerase betaDNA ShufflingDirected Molecular EvolutionEscherichia coliFrameshift MutationIn Vitro TechniquesLightMolecular Sequence DataMutagenesisPeptidesPolymerase Chain ReactionProtein EngineeringScattering, RadiationSequence Homology, Amino AcidJournal ArticleResearch Support, Non-U.S. Gov't

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Kenji Kashiwagi, Yasuhiro Isogai, Kei-ichi Nishiguchi, Kiyotaka Shiba. 2006-01-13. Frame shuffling: a novel method for in vitro protein evolution.. https://doi.org/10.1093/protein%2Fgzj008

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