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Activated CD4+CD25+ T cells selectively kill B lymphocytes.

Dong-Mei Zhao·Angela M Thornton·Richard J DiPaolo·Ethan M Shevach
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Abstract

The suppressive capacity of naturally occurring mouse CD4+CD25+ T cells on T-cell activation has been well documented. The present study is focused on the interaction of CD4+CD25+ T cells and B cells. By coculturing preactivated CD4+CD25+ T cells with B cells in the presence of polyclonal B-cell activators, we found that B-cell proliferation was significantly suppressed. The suppression of B-cell proliferation was due to increased cell death caused by the CD4+CD25+ T cells in a cell-contact-dependent manner. The induction of B-cell death is not mediated by Fas-Fas ligand pathway, but surprisingly, depends on the up-regulation of perforin and granzymes in the CD4+CD25+ T cells. Furthermore, activated CD4+CD25+ T cells preferentially killed antigen-presenting but not bystander B cells. Our results demonstrate that CD4+CD25+ T cells can act directly on B cells and suggest that the prevention of autoimmunity by CD4+CD25+ T cells can be explained, at least in part, by the direct regulation of B-cell function.

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AnimalsB-LymphocytesCD4-Positive T-LymphocytesCell Culture TechniquesCell DeathCell DivisionCoculture TechniquesFemaleFlow CytometryKineticsMiceMice, Inbred BALB CMice, Inbred C57BLReceptors, Interleukin-2Journal Article

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Dong-Mei Zhao, Angela M Thornton, Richard J DiPaolo, Ethan M Shevach. 2006-01-17. Activated CD4+CD25+ T cells selectively kill B lymphocytes.. https://doi.org/10.1182/blood-2005-11-4502

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